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Figure 5


Fig. 5. The lateral lineage covered by multiple enhancer trap GAL4 lines. (A) Schematic drawing of antennal lobe neurons labeled by different enhancer trap GAL4 lines in lAL lineage. (B-O) Composite confocal images of lAL neuroblast clones labeled by dual-expression-control MARCM and MARCM with different GAL4 lines. The name of each GAL4 driver is listed on the right (magenta text). (D,G,J,M) The single confocal images of GAL4 drivers (magenta) in the presence of LG-GH146 (yellow). (B-B'',E-E'',H-H'',K-K'',N-N'') Single confocal images of Nb clones labeled by the dual-expression-control MARCM with GAL4 drivers (B',E',H',K',N') and tubP-LG (B'',E'',H'',K'',N'') counterstained with the cell marker Acj6 (cyan). B, E, H, K and N are merged from B',B'', E',E'', H',H'', K',K'' and N',N'', respectively. (C,F,I,L,O) The morphologies of the neuroblast clones (magenta) labeled by MARCM with different GAL4 drivers and counterstained with nc82 (blue). Note the intensified DL3 glomerulus labeled by GAL4-NP6115 (L) and the thin tract that projects outward AL in the acj6-GAL4-labeled neuroblast clones (O). Abbreviations: GC, great commissure; in ant con, inter antennal connective; oACT, outer antennocerebral tract. Scale bars: 20 µm.





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