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Fig. 3. Identification of a Dlx-regulated enhancer upstream of
Gbx2. (A) Evolutionary conservation of the genomic
sequence upstream of Gbx2 analyzed using 30-way multiz alignment
(Blanchette et al., 2004).
Image generated using University of California Santa Cruz Genome Browser.
Blue, degree of conservation among mammals; black vertical bars, conservation
in each species as indicated; red bar, the Gbx2 enhancer used for the
reporter assay in C; black box, coding region of an exon; white box,
untranslated region. (B) ClustalW alignment
(Larkin et al., 2007) of the
0.6 kb region of the Gbx2 enhancer conserved down to chicken.
*, conserved nucleotides; putative Dlx-binding sites are
highlighted in orange. (C) Results of the luciferase reporter
activation assay. pGL, minimal promoter-reporter plasmid without an enhancer;
Gbx2en, pGL plasmid with mouse Gbx2 enhancer; - Dlx5, co-transfected
with empty expression vector; + Dlx5, co-transfected with Dlx5 expression
vector.
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