DEV021097 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1 Failed development of corneal endothelium in Cited2^−/− embryonic eyes. H&E staining revealed that the corneal endothelium apparent in wild-type littermate controls at 15.5 dpc (arrowhead in A) was missing in Cited2^−/− eyes at 15.5 dpc (arrow in B).

• Supplemental Figure S2 -

  Fig. S2. Smaller lens in Cited2^−/− embryonic eyes. H&E staining revealed that compared with wild-type littermate controls at 10.5 dpc (A), Cited2^−/− embryos displayed a smaller lens pit (arrow in B). The lens remained small (D) and retinal folding was also detected (arrow in D) at 18.5 dpc in Cited2^−/− eyes, as compared with Cited2^+/+ littermate controls (C).

• Supplemental Figure S3 -

  Fig. S3. Histological examination of eye sections from Cited2^+/-;Hif-1a^flox/flox;Le-Cre^-, Cited2^+/-;Hif-1a^flox/flox;Le-Cre⁺, Cited2^+/+;Hif-1a^flox/flox;Le-Cre⁻ and Cited2^+/+;Hif-1a^flox/flox;Le-Cre⁺ mice. H&E staining and histological examination of serial eye sections revealed normal eye development in Cited2^+/−;Hif1a^flox/flox;Le-Cre⁻ (A) and Cited2^+/−;Hif1a^flox/flox;Le-Cre⁺ (B) at 15.5 dpc and in Cited2^+/+;Hif1a^flox/flox;Le-Cre⁻ (C) and Cited2^+/+;Hif1a^flox/flox;Le-Cre⁺ (D) at 17.5 dpc.

• Supplemental Figure S4 -

  Fig. S4. Immunostaining to examine AP2α expression in developing lens at 13.5 dpc. Primary antibody against AP2α (Developmental Studies Hybridoma Bank) was used and the staining was revealed by DAB. An appreciable level of AP2α was detected in both Cited2^+/+ (A) and Cited2^−/− (B) lens epithelial cells.