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Figure 2


Fig. 2. Formation of the lens stalk in Cited2-/- eyes. (A-F) Histological examination was performed after Hematoxylin and Eosin (H&E) staining of serial paraffin sections of mouse embryo heads. Compared with wild-type littermate controls at corresponding stages (A,C,E), fusion of the lens to the surface ectoderm was detected at 11.5 dpc (arrow in B) and the resultant lens stalk persisted throughout development as shown in representative pictures from 15.5 (arrow in D) and 18.5 dpc (arrow in F) in Cited2-/- eyes. (G,H) E-cadherin immunostaining was performed on sections from 13.5 dpc. In contrast to the expression in corneal epithelium and lens epithelial cells in the wild-type littermate control (G), positive E-cadherin staining for lens stalk was revealed in Cited2-/- eyes (arrow in H).





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