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Figure 4


Fig. 4. Hyaloid hypercellularity and aberrant vasculature in Cited2-/- eyes. (A-D) Histological examination was performed after H&E staining of serial paraffin sections of mouse embryo heads. The analysis revealed hyaloid hypercellularity and aberrant vasculature in Cited2-/- eyes at 15.5 (arrow in B) and 18.5 (arrow in D) dpc, in comparison to normal intraocular vasculature in wild-type littermate controls at 15.5 (A) and 18.5 (C) dpc. (E-H) The abnormal vasculature was further confirmed by immunostaining Cited2-/- eye sections at 15.5 dpc for endothelial cells by CD31 (arrow in F) and endothelial and angioblast cells by VEGFR2 (arrow in H), as compared with normal expression patterns for CD31 (E) and VEGFR2 (G) in wild-type controls. (I) Vegf mRNA expression was increased in Cited2-/- lens (n=4) compared with the wild-type littermate control (n=4), as quantified by real-time PCR analysis.





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