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Fig. 1. Late stage Fgf9-/- embryos were disproportionately
shortened when compared with littermate controls. (A) Whole-mount
photograph of dissected gastrointestinal tracts of E18.5
Fgf9-/- and Fgf9+/- littermates. The
small intestines are indicated by the broken yellow boxes and the site of
cecal development by the yellow arrows. The colons are towards the right of
the small intestines. (B) Quantification of the mean small intestinal
lengths (±s.d.) of E12.5, E14.5, E16.5 and E18.5
Fgf9-/- embryos normalized to control littermates (100%).
Controls are wild-type and Fgf9+/- littermates;
n=5-7 embryos/group. An asterisk indicates the difference of the
means is statistically significant (when compared with controls at the same
time point) by Student's t-test (P<0.01). (C)
Quantification of the mean small intestinal calibers measured at the mid-point
of the proximal third, middle third and distal third (±s.d.) of E18.5
control and Fgf9-/- embryos. No statistically significant
differences were observed between these two groups. (D) Hematoxylin and
Eosin stained cross-section of control and Fgf9-/- E18.5
proximal small intestines showing premature formation of crypt-like structures
in Fgf9-/- mice that were not observed in controls (shown
at higher magnification on the right). (E) No other morphological
differences were noted, including restriction of BrdU-positive cells to the
intervillus epithelium. (F) Quantification of mean number of goblet and
enteroendocrine cells per 100 epithelial cells (±s.d.) in proximal,
middle and distal small intestines of E18.5 control and
Fgf9-/- mice. No statistically significant differences
were observed between these two groups. (G) Immunohistochemically
stained sections of E18.5 control (top) and Fgf9-/-
(bottom) littermates showing that I-fabp (left panels) in the proximal small
intestines and Ilbp (right panels) in the distal small intestines were
expressed at detectable levels in villus enterocytes of controls but not
Fgf9-/- embryos. Scale bars: 100 µm in D; 30 µm in D
(higher magnification), E,F.
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