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Fig. 2. Fgf9 regulates mesenchymal fibroblast proliferation. (A)
Hematoxylin and Eosin stained section (left) and color-coded cartoon (right)
showing micro-anatomical regions of a control E18.5 small intestine.
(B) Immunohistochemistry of BrdU stained sections of control (left) and
Fgf9-/- (right) E18.5 proximal small intestines. The
broken red lines indicate the boundaries between the epithelium, mesenchyme
and muscle layers. (C) Quantification of the means (±s.d.) of
the proportion of S-phase cells (as indicated by BrdU incorporation) in the
epithelium, mesenchyme and muscularis propria for E14.5, E16.5 and E18.5 for
control and Fgf9-/- embryos. Statistically significant
differences between the two groups (n=4 embryos/group) were
determined by a Student's t-test (**P<0.01;
*P<0.05). (D) Quantification of the mean percent
BrdU incorporation (±s.d.) in fibroblasts (prolyl 4-hydroxylase
positive) and endothelial cells (Pecam1 positive) from the mesenchyme of E18.5
control and Fgf9-/- small intestines. Statistically
significant differences between the two groups were determined by comparison
of SEMs using Student's t-test; **P<0.01;
n=4 mice/group). Scale bars: 30 µm.
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