|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 2. Characterization of β-catenin-activated hES cells.
(A) The 
Nβ-cateninER cells cultured with or without 4OHT
for the indicated time periods were subjected to immunostaining with anti-KDR
and anti-CXCR4 antibodies, and analyzed by FACS. The gated region denoted the
cells scored as antigen-positive (the percent cells scored positive is shown
in each panel). ES, unstimulated Nβ-cateninER cells. (B)
Developmental potentials of β-catenin-activated cells towards mesoderm
lineage. RT-PCR analysis of endothelial cell-specific markers at different
time points: the Nβ-cateninER cells were cultured with 4OHT for 3
days (day 3), and further cultured with vascular endothelial growth factor
(VEGF) for additional 3 days (day 6). The transgenic cell lines derived from
different ES cell lines are shown (K1βcatER derived from KhES-1 cells,
K3βcatER derived from KhES-3 cells). (C) Morphology of
endothelial-like cells derived from β-catenin-activated cells (day 6).
(D) Capillary tube-like networks formed on matrigel for 24 hours after
culturing endothelial-like cells derived from β-catenin-activated cells.
Scale bar: 100 µm.
|
