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Fig. 8. RNAi-induced loss of BMPR1A, by reducing MMP2 expression,
attenuates apoptosis of human pericytes (HBVPs). (A) Gelatin
zymography using conditioned media of HBVPs in response to serum deprivation.
Gelatin zymograms of conditioned media (top) with densitometric analysis
(beneath) to assess MMP2 activities in SiBMPR1A-transfected (SiBR1A) as
compared with SiControl-transfected (SiC) cells. Bars represent
mean±s.e.m. of densitometric values of SiBR1A MMP2 forms normalized to
the corresponding SiC value. n=7-8 for pro and n=3-4 for
active MMP2. *P<0.05 versus SiC. (B) Reduced
MMP2 activity in HBVPs transfected with MMP2 RNAi. Gelatin zymogram of
conditioned media (top) and densitometric values (beneath) of MMP2 in HBVPs
transfected with SiMMP2. Bars represent mean±s.e.m. of densitometric
values of SiBR1A MMP2 forms normalized to SiC value (n=4).
***P<0.001. (C) Apoptosis of HBVPs in response
to serum deprivation using caspase 3/7 assay. Apoptosis in HBVPs transfected
with SiC, SiBR1A or SiMMP2 was induced by serum-deprivation for 24 hours and
assessed by a caspase 3/7 luminescent assay. Bars represent mean±s.e.m.
of arbitrary luminescent values (n=6-9).
***P<0.001 versus SiC.
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