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Fig. 3. Division axes of p2 intermediate progenitors are not fixed.
(A,B) Confocal stacked images of vsx1-GFP sibling cells
immediately after division. Embryos were analyzed at around 16-17 hpf. (A1-A4)
Lateral views. Dorsal is towards the top. (B1-B4) Dorsal views. Superficial is
towards the top. (C) Schematic of the method for division axis
measurements. For each division, a line connecting the centers of the two
sister cell (projection of division axis) was drawn. The angle of this line to
the horizontal line (lateral views) or to the midline (dorsal views) was
measured. (D,E) Quantification of the division angle.