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Fig. 4. Non-cell-autonomous effect of the Ret- mutation on
the colonisation of E8.5 by Ret+ ENS progenitors. Whole-mount
X-Gal staining of E8.5+3+7 guts from Ret+/- (A) and
Ret-/- (B) mouse embryos grafted with
Ret+ cells from the intestine of E11.5 Rosa26bgeo embryos.
Contrary to control gut (A), no β-gal+ cells were detected
beyond the anterior foregut of Ret-deficient gut (B). Representative images of
Ret+/- (C) and Ret-/-
(D) E8.5+3 embryos grafted at E8.5 with Ret+ cells isolated
from the intestine of E11.5 embryos. Grafted embryos (three homozygous mutant
and 12 heterozygous or wild-type) were cultured for 3 days and then processed
for β-gal histochemistry. Arrows in C and D point to
β-geo+ cells in the foregut (stomach and duodenum anlage). st,
stomach; mg, midgut.
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