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Figure 1


Fig. 1. Molecular analysis of maize sid1. (A) Amino acid alignment of maize SID1 (top) and IDS1 (bottom). Black line marks the AP2 domain. (B) Bayesian phylogram of DNA sequences of the AP2 domains of all MIR172-targeted genes from maize and rice. The Arabidopsis AP2 gene was used as the outgroup. (C) Genomic structure of the maize sid1 gene. Boxes represent exons, dark boxes represent the AP2 domain, and triangles represent Mutator transposon insertions. (D) RT-PCR of sid1 (top) and actin (bottom) from different tissues and tassels of ts4 mutant alleles. (E) Location of cleavage sites within sid1 from B73 ears (top) and ts4-TP ears (bottom). The number of clones cleaved at each different position within the microRNA binding site over the total number of clones analyzed is indicated; those in parentheses to the right indicate the number of clones cleaved 3' of the microRNA binding site. (F)3 RNA gel blot using 1 µg of poly(A)+ RNA from 0.5 cm tassels. Arrowheads mark positions of the 28S and 18S ribosomal RNAs. (G) RNA gel blot using 1 µg of poly(A)+ RNA from 0.5 cm ears. The 3' ends of ids1 and sid1 outside of the coding region for the AP2 domain were used as probes.





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