spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 2


Fig. 2. phyl mediates G2-M transition by positively regulating stg mRNA expression. (A,B) stg mRNA expression pattern at the AWM of wild-type (A) and phyl2/phyl4 (B) wing disks at 2-4 hours APF. Arrows indicate the stg-positive SOPs. In phyl mutants, stg expression is significantly reduced in AWM SOPs (B). (C-F) Ectopic expression of stg rescues the defects in G2-M transition. (C) Percentage of cell division of thoracic SOPs at 22-24 hours (column 1 and 2) and 24-26 hours (column 3-4) APF. SOP division defect is strongly rescued when stg was added back transiently to phyl mutant pupae by heat-shock treatment (lanes 2 and 4). *P<0.0005, n=4-9 thoraces. The numbers in the parenthesis are the number of SOP scored. (D-F) Images of pupal thoraces stained with anti-Sens antibody (green). (D) Wild-type. (E) phyl2/phyl4; hs-Gal4/+ pupa with two pulses of heat-shock treatment. (F) phyl2/phyl4; hs-Gal4 UAS-stg/+ pupa with two pulses of heat-shock treatment.





Right arrow Return to article