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Fig. 1. Bowl accumulates in the PE and is absent from the DP. Spatiotemporal
expression pattern of Bowl (red), wg-lacZ (green) or
odd-lacZ (green). Optical sections at the plane of the PE (A,C,E) and
DP (B,D,F). (A,B) 48 hours AEL; (C,D) 72 hours
AEL; (E,F) 96 hours AEL. wg-lacZ was detected in the DP
in a wedge of ventral cells (arrow in B). wg was upregulated along
the DV compartment boundary (arrows in D and F). Bowl accumulated broadly in
the PE (A,C,E) and was absent from the DP (B,D,F), except for the lateral
margins of the notum (arrowheads in D and F). (G,H) 120-144
hours AEL; Bowl accumulated in the anterior margin of the PE (asterisks in G
and H) and the lateral margins of the notum (arrowheads in H). However, Bowl
was downregulated in the medial and posterior regions of the PE. (I)
Everting discs; Bowl accumulated in the ventro-anterior hinge (asterisk) and
in the lateral margins of the notum (not shown in this image). (J)
odd-lacZ was detected in a similar pattern at this stage (arrowheads
indicate the lateral margins of the notum, asterisks the ventro-anterior
hinge). (K) Adult wings; odd-lacZ was detected in
ventro-proximal hinge nuclei. (L) A schematic diagram illustrating the
regulatory interactions linking drm, lines and bowl.