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Fig. 2. The Lines and Bowl proteins accumulate in a reciprocal pattern across
the PE and the DP. Clones expressing (A-C) Myc-Lines (weak insertion), (D)
Flag-Bowl, (F) Drm or (G) Lines (strong insertion) were marked with nuclear
GFP reporter, which accumulates to high levels in nuclei and to lower levels
in the cytoplasm. (E) lines mutant clones were GFP-negative. Arrows
in A-C,E-G indicate magnified areas shown in insets. (A-B'')
Optical sections at the plane of the PE; Myc-Lines was enriched in the
cytoplasm in the lateral margins of the PE (A-A''), and in the simple
squamous PE (B-B''). DP clones below the PE are visible in these images.
(C-C'') However, Myc-Lines was enriched in nuclei in the DP.
(D,D') Flag-Bowl accumulated only in clones generated in
the PE (arrow in D') and the ventro-anterior hinge (arrowhead in
D'). Flag-Bowl failed to accumulate in clones generated in the DP. Bowl
was stabilized in lines mutant cell clones (E-E'') and in
drm expressing clones (F-F'') in the DP, and lost from
the lines-expressing cell clones generated in the lateral margins of
the PE (G-G'', arrow in G) and in the squamous PE (not shown).
Clones were induced at 24-48 h AEL in A-D and at 72-96 h AEL in E-G.
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