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Fig. 5. Ectopic lines expression can completely transform the PE into
DP, induce formation of secondary wings, and disrupt notal growth. (A,B)
Wild type. (C-H) lines expressing clones (green) induced at the early
first instar. Dapi (blue), Tsh (yellow), Wg, Nub or Al (red). (A) Tsh
is excluded and Nub is restricted to the pouch and distal hinge. (B) Wg
demarcates the pouch, the proximal and the distal hinge, and the medial region
of the notum. (C) Large patches of lines-expressing cells in
the DP had no effect on wing development. (D) Large patches of
lines-expressing clones in the PE completely transformed the PE into
DP. Arrowheads indicate the z-section shown in insets. Tsh and Nub
were symmetrically expressed in both layers of the disc. (E,F)
Smaller clones led to formation of secondary wings (arrows in right panels
indicate secondary wings, arrowheads indicate Wg expression along the wing
margin). These clones localized to the central region of the secondary wings.
Large arrowheads indicate plane of z-section shown in insets. (F) In
a subset of discs, the notum was reduced or absent. Asterisk indicates the
loss of Wg expression in the presumptive notum. (G) Ptc-GAL4
UAS-GFP; GFP expression was detected along the AP compartment boundary in
the DP and in the PE (marked by apposing arrows); Al marks the anterior part
of the pouch. (H) Ptc-GAL4 UAS-GFP UAS-Lines; the
Ptc-expressing cells in the PE adopted DP fate and permitted
formation of secondary wings. Al and GFP were co-expressed in a subset of
pouch cells.
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