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Fig. 1. Abrogation of Mil function facilitates anterior migration of the
presumptive prechordal plate. In situ hybridisation
(A-H,O,P) of dlx3 (anterior border of the neural
plate), hgg1 (anterior prechordal plate) and ntl (notochord)
at tailbud stage, (I-L) of dlx3 and hlx1 (posterior
prechordal plate) at tailbud stage, and (M,N) of spry4
(presumptive mesoderm) at 60% epiboly. (A-D) Dorso-animal views, (E-H) lateral
views, (I-L) dorso-animal views and (M-P) dorsal views of wild-type (WT)
embryos (A,E,I,M), WT embryos injected with 4.3 ng mil-MO (B,F,J,N),
slb embryos (C,G,K,O) and slb embryos injected with 4.3 ng
mil-MO (D,H,L,P). In mil-MO-injected embryos, anterior
migration of the presumptive prechordal plate was promoted (B,F), as compared
to WT (A,E), with regards to relative positions of the prechordal plate in
relation to the anterior border of the neural plate (A-D), and to angles
between the prechordal plate and the tailbud, indicated by arrowheads (E-H).
Injection of mil-MO rescued the reduced migration of the anterior
prechordal plate in the slb embryo (C,D,G,H), but did not alter the
wider notochord (O,P, indicated by asterisks). By contrast, the altered
position of the posterior prechordal plate correlated with that of the
anterior prechordal plate (K,L). (M,N) The enhancement of migration was not
observed in the lateral mesoderm (brackets), but was specific to the
prospective prechordal plate (arrow).
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