spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 1


Fig. 1. Mesoderm defects in chato embryos. Wild-type (wt) (A,C,E,G) and chato mutant (B,D,F,H) mouse embryos were assayed by in situ hybridization with markers expressed in head mesenchyme/lateral plate mesoderm/somitic mesoderm (Twist1; A,B, dorsal and ventral views, respectively), somites (Meox1; E,F, ventrolateral views) and cardiac mesoderm (Nkx2.5; G,H, ventral views). Staining for β-galactosidase activity from a Nodal-lacZ reporter labeled lateral plate mesoderm and node of wild-type (C) and chato mutant (D) embryos (lateral views). Thirty-three percent of chato mutants (n=184) had condensed somites that appeared narrow and laterally extended (F). In 52% of chato embryos (n=184), somites were not clearly discernible morphologically, but somite markers Twist1 and Meox1 marked some imperfectly shaped somites. Only 15% of chato mutants showed normal somites. Arrowheads in A,B point to head mesenchyme. Brackets in C,D highlight the different width of the lateral plate mesoderm in wild-type and chato mutant embryos. Brackets in E,F highlight the different width of the somites. Arrowheads in H mark the cardiac mesoderm in chato mutants. LPM, lateral plate mesoderm; som, somites.





Right arrow Return to article