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Fig. 5. Failure of convergent extension in the definitive endoderm of
chato mutants. (A-D) Wild-type (A,B) and chato
mutant (C,D) 8-somite stage mouse embryos hybridized with Ttr probes
to highlight extraembryonic visceral endoderm (blue) and definitive endoderm
(exterior layer of embryonic tissues in white). ext, white extraembryonic
tissue. (A,C) Lateral views; (B,D) anterior views. (E-G) Plots of (E)
wild-type (blue) and chato (red) definitive endoderm length (µm),
(F) wild-type (green) and chato (red) definitive endoderm width
(µm), and (G) definitive endoderm length-to-width ratio (LWR) in wild-type
(gray) and chato mutant (red) embryos. Error bars indicate s.d.
*P<0.05, **P<0.01. (H)
Length and width average measurements ±s.d. in µm. The number of
embryos analyzed for each stage is indicated (# embryos); na, not assayed.
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