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Figure 7


Fig. 7. Proliferation in definitive endoderm. Cryosections of wild-type (A,C) and chato mutant (B,D) mouse embryos at different embryonic stages were labeled with anti-E-cadherin (red) and phospho-histone H3 (green) antibodies. Mitotic cells (green) in the definitive endoderm (highlighted in red by localization of E-cadherin) are indicated by arrowheads. E-cadherin is also present in E7.5 epithelia (ep), embryonic surface ectoderm (se) and extraembryonic visceral endoderm (VE, dashed line). Proliferation of mesoderm and epithelial tissues was not significantly different between wild-type (n=21 embryos/380 sections) and chato mutant (n=3 embryos/56 sections) embryos at these stages.





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