DEV024588 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Fins recover lost structures after restoration of Fgf signaling. The same hsp70:dn-fgfr1 fin pictured at day 60 of Fgfr inhibition (left), and after 30 days of recovery at room temperature (right). The majority of fin rays recover ostensibly all lost structures. Inset shows region from which new growth was initiated after Fgf signaling. Arrowheads indicate the start of new tissue.

• Supplemental Figure S2 -

  Fig. S2. Injuries to distal fin structures are rare under laboratory conditions. In animals imaged every 2-3 days over 14 to 24 days, we found that injuries and loss of distal bone were rare (2 events in 13 animals), and were rapidly regenerated (arrows). Scale bar: 100 µm.

• Supplemental Figure S3 -

  Fig. S3. Markers of facultative regeneration are expressed at low levels in uninjured fins. The blastemal regulators mkp3, msxb, fgf20a and mps1 are all robustly expressed in samples from regenerating fins, as assessed by RT-PCR. Expression of these factors is reduced, but detectable, in the uninjured fin. The control, β-actin1, is found at similar levels in samples taken from both uninjured and regenerating fins.