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Fig. 4. Scanning mutation analysis of in vitro binding of ECudA to
oligonucleotide B. Scanning mutation analysis of ECudA binding was
performed as described in the text using oligonucleotide B. The sequence of
the inserted mutation was GCGCGC. Each of the 11 mutant forms was used as
competitor in a band shift assay with oligonucleotide B as the probe.