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Fig. 8. Mutational analysis of the cotC promoter. (A)
Deletion analysis. A 5' to 3' deletion series with a start point
at nucleotide -659 (relative to the cap site) was constructed and fused to the
lacZ gene at a point 30 nucleotides downstream from the cotC
initiation codon. The constructs were used to prepare stable transformants in
Ax2 and cudA-null cells and analysed as pooled populations. Note the
shorter staining time used for the -659 construct. (B) Point mutation
analysis. Site-directed mutagenesis was used to generate a construct with a
5' end point at nucleotide -457 and with a structure similar to that
described in A. This construct, and the control wild-type construct, were
analysed as in A but only in Ax2 cells.
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