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Fig. 1. Core polarity gene function and the site of prehair initiation. Drosophila 32-hour pupal wings immunolabelled for actin [Ph; magenta in B-H, white in E'-H'], clonal marker (lacZ, fz-EGFP or dsh expression; green) and Fmi or Stbm (blue). Arrowheads indicate prehairs initiating at edges of mutant cells touching a mitotic clone boundary. Distal is to the right in this and subsequent figures. Diagrams illustrate sites of core polarity protein localisation (Fz/Dsh distal complex in green, Stbm/Pk proximal complex in orange) in wild type or on edges of clones, and observed sites of prehair initiation; first row of mutant cells in each clone marked with asterisks. Sites of prehair initiation in mutant cells away from clone edges are not illustrated but are located at the cell centre (except as noted in the text for the first rows of cells within stbm tissue). (A) Diagram of normal localisation of distal and proximal core polarity proteins and site of prehair initiation in wild-type wings. (B,B') fz²¹ clone. Note that prehair initiation is delayed in mutant cells away from clone edge. Normal polarity is reversed in non-mutant cells owing to the influence of the clone (Vinson and Adler, 1987). (C,C') stbm⁶ clone. Note that prehair initiation is less delayed than in fz clones and is more likely to be towards a cell edge. (D,D') stbm⁶/fz²¹ twin clone. (E,E') sha¹/fz²¹ twin clone. (F,F') stbm⁶/sha fz²¹ twin clone. (G,G') stbm⁶ clone in a fz²¹ background. (H,H') fz²¹ clone in a stbm⁶ background. Note that fz mutant cells are marked by loss of Dsh junctional recruitment (Shimada et al., 2001). Scale bar: 10 µm.

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