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Fig. 4. Expression of Cluster I genes in single-cell cDNAs and in the E14
brain. (A) Expression levels of Cluster I genes. Data from the
probe sets that were expressed in Cluster I, but not in Cluster II/III, were
used for clustering (n=175 probe sets; Welch's t-test,
two-tailed, FDR<0.1 log fold-change cut-off <-2.5). Each column
indicates one cell, and each row indicates one probe set on the microarray.
The expression levels are color-coded from red (high) to black (low). Unlike
the Cluster II/III genes (see Fig.
2), no particular pattern was seen in the Cluster I genes, except
for the I+ II- III- IV+ genes
(5730410E15Rik, Dab1). (B) Examples of in situ hybridization
of the E14 mouse brain for Cluster I genes. In all cases, signals were seen in
the VZ. The 5730410E15Rik and Dab1 genes were expressed in
both the VZ and CP (n,o), consistent with the single-cell gene expression
profiles (A). GenePaint set IDs are indicated at the bottom right of some
panels.
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