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Fig. 2. Transition of hnRNP K from predominantly nuclear to mixed nuclear and
cytoplasmic localization during neuronal development. (A-C) hnRNP K
immunofluorescence (A), DAPI-fluorescence (B) and phase-contrast (C) images of
embryonic muscle cell and neurons in dissociated cell culture. hnRNP K
immunostaining colocalized with DAPI-stained nuclei (arrowheads, A,B);
neurites and growth cones (gc) had no immunostaining (arrows in A,C).
(D,E) hnRNP K immunoperoxidase staining of sections of juvenile
frog retina (D) and spinal cord ventral horn (E). Retinal ganglion cells (RGC)
exhibited more intense cytoplasmic than nuclear staining; motoneuron staining
was both in the nucleus and cytoplasm (arrowhead, E). Arrow in E indicates
interneuron with predominantly nuclear staining.
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