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Fig. 4. Suppression of axonal outgrowth by hnRNP K MO in intact animals.
Embryos unilaterally injected with control MO (A), hnRNP K MO1 (B,D-H) or
hnRNP K MO2 (C) processed at stage 37/38 by whole-mount immunofluorescence.
(A-C) Embryos injected with control MO (A), hnRNP K MO1 (B) or MO2 (C),
and immunostained for NF-M. Broken lines separate injected (lower) from
uninjected (upper) sides. (B,C) Arrowheads on the injected side indicate
peripheral motor axons; arrowheads on the uninjected side indicate residual
wispy peripheral axons. (A) View under the fluorescence dissecting microscope;
(B,C) stacked confocal optical sections. (D1) Neuronal β-tubulin
staining in neuronal perikarya and peripheral axons of spinal cord, uninjected
side. (D2) Tubulin staining in perikarya and a few scattered fibers,
injected side. (E1-G2) Peripherin immunostaining of head (E1,E2) and
spinal cord (F1,F2,G1,G2). (E1,F1,G1) Uninjected side; (E2,F2,G2) injected
side. (E1,E2) Trigeminal ganglion neuronal perikarya (upper arrowhead) and
nerves (lower arrowhead). (F1,F2) Spinal cord with peripheral motor axons.
(G1,G2) Spinal cord neuronal perikarya. (H) Spinal cord immunostained
for HNK-1. Robust staining of neuronal perikarya and axons on the uninjected
side (below the broken line); clusters of stained neuronal perikarya
(arrowheads) and very few fibers on the injected side (above the broken line).
Scale bars in D1,E2,F1 and G2 also apply to D2,E1,F2 and G1, respectively.
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