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Fig. 2. Dlx3 expression during hf development. (A) Dlx3 expression
was detected using an anti-Dlx3 antibody in the hair matrix cells at postnatal
day 1 (P1, early anagen), P9 (full anagen) and P17 (catagen) in the telogen
bulge cells at P20 (inset, higher magnification of bulge area in box), P25
(the onset of first postnatal anagen) and P30 (first postnatal anagen).
(B) (Top row) Immunofluorescence studies on P1 hf using anti-Dlx3
(green) and anti-β-galactosidase (red) to demonstrate colocalization of
Dlx3 and lacZ expression. (Lower rows) Immunofluorescence with
anti-keratin antibodies (green): anti-K17 at P9; anti-K35 and anti-K85 at P30.
Merged images are shown with DAPI staining in the right column. ORS, outer
root sheath; IRS, inner root sheath; CU, cuticle; DP, dermal papilla. Scale
bar: 50 µm.
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