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Fig. 3. Conditional deletion of Dlx3 in the epidermis using K14cre.
(A) Gene targeting to generate the floxed-Dlx3 mice. (Left panel) LoxP
sites were inserted into the NotI site (N) between the first and
second exons of the Dlx3 gene and immediately downstream of the neomycin gene
(Neo). White and black arrowheads indicate the localization of the
oligonucleotides used in the PCR reactions. (Right panel) Recombination in the
ES cells was assessed by Southern blot analysis; WT, wild type;
Dlx3f/+, heterozygote for floxed Dlx3 allele. (B) Gross
phenotype of wild type (top) and K14cre;Dlx3f/f (bottom) mice at P9
(left) and 15 weeks (right). (C) Genotype by PCR for wild-type (WT) and
K14cre;Dlx3f/f mice showing epidermal specific recombination of the
Dlx3 allele in the K14cre;Dlx3f/f skin. (D) The specific
deletion of Dlx3 expression was detected in the K14cre;Dlx3Kin/f hf
extracts by western blot analysis. Asterisk indicates Dlx3-specific band.
(E) The specificity of Cre-mediated recombination was also determined
by immunohistochemistry on skin sections from wild-type and
K14cre;Dlx3Kin/f littermates. Dlx3 (green) expression is shown in
the matrix cells of P1 hf in the wild-type skin; however, only the
lacZ (red) expression is detected in the K14cre;Dlx3Kin/f
skin. Images are shown with DAPI staining to denote nuclear staining. Scale
bar: 50 µm.
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