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Fig. 5. Follicular signaling in the regulation of Dlx3 expression.
(A) Expression of Lef1, β-catenin and Smad1/5/8 were determined in
control and conditional knockout skin to analyze effects of absence of
epithelial Dlx3 at P1 and P9 anagen stages. (B) Colocalization of
Dlx3/lacZ with Lef1. The skin sections of wild type
(Dlx3Kin/+) at P1 were stained with by anti-Lef1, anti-phospho
Smad1/5/8 and anti-β-galactosidase antibodies (red, middle); the merged
images are shown with DAPI staining. (C) (Top) Lef1 direct binding in
vivo to the Dlx3 promoter was demonstrated by ChIP assays using Lef1
antibody. Putative Lef1-binding site in the mouse Dlx3 promoter
sequence (-160 to +15 bp) is indicated in red. CCAAT box and TATA box are
underlined. The transcriptional start site is indicated by an arrow. (Bottom
left) Gel image of ChIP assay. (Bottom right) The specificity of the ChIP
assays, determined by both control IgG antibody and a set of PCR primers for
Gapdh. Scale bars: 50 µm.
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