DEV019919 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. HH signaling to the myocardium and perivascular cell differentially regulates Vegf ligand expression. (A-F) Whole-mount in situ hybridization for Vegfa (A,B), Vegfb (C,D) and Vegfc (E,F), showing that, compared with controls (A,C,E), Smo^{mlc2v; Dermo1} CKO hearts (B,D,F) display decreases to Vegfa, Vegfb and Vegfc expression. (G-L) Cryosections of hearts stained with in situ probes to Vegfa (G-I) and Vegfb (J-L). Although control hearts (G,J) contain expression of Vegfa and Vegfb in both cardiomyoblasts (asterisk) and perivascular cells (arrowhead), Smo^mlc2v CKO hearts (H,K) express these transcripts in only perivascular cells (arrowhead). By contrast, Smo^Dermo1 CKO hearts (I,L) express Vegfa and Vegfb in only the cardiomyoblast (asterisk). (M-P) Whole-mount in situ hybridization for Vegfc demonstrating that, compared with controls (M), Smo^mlc2v CKO hearts (N) express similar levels of Vegfc. Compared with controls (O), Smo^Dermo1 CKO hearts (P) display diminished Vegfc expression.