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Figure 2


Fig. 2. GATA4 and GATA6 are required for Wnt11 expression and heart muscle differentiation in Xenopus. RT-PCR analysis of Nkx2-5, Wnt11, MLC2 and TnIc gene expression in embryonic explants and whole embryos at control stages 24 and 32, as indicated. (A) Animal cap explants injected with RNA encoding Activin (Act, 50 fg) to induce cardiogenesis and in combination with morpholinos targeting GATA4 or GATA6 (G4MO, G6MO), as indicated. (B) Ventral marginal zone (VMZ) explants injected with Dickkopf-1 mRNA (Dkk-1, 800 pg) to induce ectopic cardiogenesis and together with G4MO, or G6MO as indicated. (C) Analysis of gene expression in whole embryos injected with G4MO or G6MO as indicated. Note that morpholino-mediated inhibition of either GATA4 or GATA6 reduces Wnt11 and cardiogenic marker gene expression, but that inhibition of both GATA4 and 6 abolishes them in all three assays.





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