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Fig. 1. Expression patterns of Xenopus laevis Irx genes during
pronephros development. Embryos are shown in lateral views (except when
indicated); red arrowheads indicate the kidney territory. (A-D)
Expression pattern of Irx1 at indicated developmental stages. At
mid-(A) or late (B) neurula, Irx1 is detected in the dorsal
pronephric territory. Insets show pronephric territory of an embryo
double-stained for Lim1 or Pax8 (blue) and Irx1
(purple). Irx1 expression is restricted dorsally. During tailbud (C)
or tadpole (D) stages, Irx1 expression shifts to a more ventral
region that will form the intermediate tubule. Inset in C indicates an embryo
double-stained for Pax8 (blue) and Irx1 (purple). Note the
ventral position of Irx1 in the future intermediate tubule. Inset in
D indicates a higher magnification of the pronephric Irx1 territory.
Note the expression of Irx1 in the migrating ventral mesoderm (blue
arrowheads). (E-H) Irx2 shows an expression pattern similar to
that of Irx1, although it is not expressed in ventral migrating
mesoderm. (I-L) Spatial distribution of Irx3 mRNA. (I) At
mid-neurula, Irx3 mRNA is detected in a broad domain that contains
most of the pronephric territory. Inset indicates pronephric territory of an
embryo double-stained for Lim1 (blue) and Irx3 (purple). (J)
At late neurula/early tailbud stages, Irx3 becomes restricted to the
ventral pronephric territory. Inset indicates Irx3 ventral
restriction in an embryo co-stained for Pax8 (blue). (K,L) From
tailbud stages, Irx3 expression is detected in the intermediate
tubule. Inset in K indicates embryo double-stained for Pax8 (blue)
and Irx3 (purple). Inset in L indicates high magnification of the
pronephric Irx3 territory. (M,N) Lateral view (M) and
transverse section (N) of late neurula embryos showing Lim1 (blue)
and Irx1 (purple) expression. Irx1 expression is restricted
to the dorsal pronephric anlage. (O,P) Double staining for
Sglt1k (green) and Irx1 (red) in tailbud (O) or tadpole (P)
embryos. Insets indicate single Irx1 red channel. The Irx1
expression domain is located just distal to that of Sglt1k.
(Q,R) Lateral view (Q) and transverse section (R) of late
neurula embryos showing Lim1 (blue) and Irx3 (purple)
expression. There is initial broad expression of Irx3 in most of the
pronephric anlage (Q) and a later restriction to the ventral pronephros (R).
(S,T) Double staining for Nkcc2 (green) and
Irx3 (red) in tailbud (S) or tadpole (T) embryos. Insets show single
Irx3 red channel. The expression domains of both genes largely
overlap, but the Irx3 domain extends proximally into the proximal
tubule, whereas Nkcc2 extends distally into the distal tubule.
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