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Fig. 4. Overexpression of Irx genes in Xenopus expands the pronephric
territory. Embryos are shown in lateral views (except when indicated).
Embryos were injected in a single blastomere (V2.2) at the 8- to 16-cell stage
and lacZ mRNA was used as linear tracer. Neurula (A-P) or tadpole
(Q-R) embryos injected with different mRNAs. Transverse sections of tadpole
embryos are shown in S,T. (A-H) Overexpression of 300 pg of
MT-Irx1-GR (A-D) or MT-Irx3-GR (E-H) mRNAs expands
(arrowheads) ventrally the expression of Lim1 (A,B,E,F) and
Pax8 (C,D,G,H) upon addition of dexamethasone (Dex) at stage 14,
whereas no effect was observed in the absence of Dex (not shown). (I-P)
Embryos co-injected with a mix of Irx1 and Irx3 MOs and
MT-Irx3-GR mRNAs show strong downregulation of Lim1 (I,J;
arrowhead) and Pax8 (K,L; arrowhead) in the absence of Dex. (M-P)
This phenotype is rescued upon addition of hormone at stage 14. (Q-T)
Tadpole embryos injected with MT-Irx1-GR mRNAs and Dex treated at
stage 14 show enlarged kidneys (Q,R; arrowhead), as determined by staining
with the 3G8 antibody. (S) Transverse section of a similarly injected embryo.
(T) The same section treated with propidium iodide for nuclear staining. The
control and the enlarged pronephros show the same cellular morphology.
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