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Fig. 6. Irx gene loss of function kidney defects are partially rescued by
increased Smad1 activity in Xenopus. Embryos are shown in lateral
views and red arrowheads indicate the kidney territory. Embryos were injected
in a single blastomere (V2.2) at the 8- to 16-cell stage and lacZ
mRNA was used as linear tracer. (A,B) Injection of 500 pg of
Smad1GR mRNA, upon addition of dexamethasone (Dex) at stage 14,
expanded Pax8 expression. No effect was observed in the absence of
hormone (not shown). (C-F) In embryos co-injected with 500 pg of
Smad1GR mRNA and 4 ng of each Irx1 and Irx3 MOs
Pax8 expression was downregulated (C,D) or rescued (E,F) in the
absence or presence of Dex, respectively. (G,H) Depletion of
Irx1 and Irx3 impaired Bmp7 expression.
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