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Fig. 7. Injection of different doses of Irx1 or Irx3 MOs reveal an
early and a late requirement of this gene during pronephric development in
Xenopus. Embryos are shown in lateral views and red arrowheads
indicate the kidney territory. Embryos were injected in a single blastomere
(V2.2) at the 8- to 16-cell stage and lacZ mRNA was used as linear
tracer. (A-D) Injection of low doses (4 ng) of Irx1 MO had
little effect on Sglt1k expression (A,B) but downregulated the
proximal domain of Nkcc2 (C,D). (E-L) Injection of low doses
(4 ng) of two different Irx3 MOs downregulated the distal expression
of Sglt1k (E,F,I,J) and the proximal domain of Nkcc2
(G,H,K,L). No effect was observed in the duct, as determined by Gata3
expression (E,F,I,J). (M-P) Injection of high doses (8 ng) of
MOIrx3.2 strongly downregulated Sglt1k (M,N) and
Nkcc2 (O,P). Most injected embryos were malformed, as shown in O,P.
Nevertheless, a few displayed normal morphology, like that shown in M,N.
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