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Fig. 3. sq181 mutant hepatoblasts are impaired in proliferation and
subsequently undergo cell apoptosis that leads to a liverless phenotype.
(A,B) Immunostaining using an anti-P-H3 antibody on (A)
wild-type (wt) and (B) mutant (mu) zebrafish embryos at 30 hpf.
(C,D) TUNEL assay of cell apoptosis in (C) wild-type and (D)
mypt1sq181 mutant embryos at 38 hpf. The white dotted line
circles the liver primordium and the adjacent endoderm region.