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Figure 6


Fig. 6. The mypt1sq181 mutation alters actin assembly in LPM and endoderm cells and causes a posterior shift of the liver primordium. (A,B) Cross-sections from wild-type (WT) and mutant (mu) zebrafish embryos in the Tg(gutGFP)S584 background (green) stained with phalloidin (red). (C-F) High-magnification views of boxed regions in A (C,D) and B (E,F) showing abnormal aggregations of actin filaments (arrows) and cell organization in the mutant endoderm (C versus E) and LPM (D versus F) cells. (G,H) WISH using insulin (for islet, blue arrowheads) and hhex (for liver, arrow) probes, and F59 antibody (for somites, horizontal black lines) on mutants (mu) and wild-type (wt) embryos.





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