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Fig. 1. LH reduces gap junction permeability between cumulus and mural granulosa
cells. Determined by injecting Alexa Fluor 350 into the oocyte and imaging
its diffusion within the follicle at 20 minutes after injection.
(A,B) No LH. (C,D) LH applied 70 minutes before
injecting Alexa Fluor 350. Upper panels show scanning transmission images;
lower panels show Alexa Fluor 350 distribution. In A and B, Alexa Fluor 350
diffused throughout the mural granulosa cells, whereas in C and D, the Alexa
Fluor 350 spread into the cumulus cells, but little or none was seen in the
mural granulosa cells. (E) Regions of the follicle used for the
measurements shown in F. The dashed lines indicate the approximate border
between the cumulus mass and the antral space. (F) Ratio of
fluorescence intensity in the mural granulosa cells to that in the inner
cumulus cells, as a function of LH treatment time. Measurements were made at
20 minutes after injecting Alexa Fluor 350 into the oocyte. Bars show
mean±s.e.m., and the numbers in parentheses indicate the number of
follicles tested at each time point (0.5 hour=33-40 minutes, 1 hour=64-75
minutes, 2 hours=124-140 minutes, 5 hours=4.5-5.7 hours).
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