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Fig. 2. LH reduces gap junction permeability between mural granulosa cells.
Determined by fluorescence redistribution after photobleaching of Alexa Fluor
488. (A) No LH. (B) LH applied 58 minutes before photobleaching.
For A and B, a rectangular region was photobleached for 4.5 seconds, between
the images indicated by the arrow. Each column shows images before and at
various times (in seconds) after the end of the bleach. (C,D)
Fluorescence intensity in the bleached region as a function of time, for the
images shown in A and B. (E) Percent recovery of fluorescence intensity
in the bleached region during the first minute after the bleach, as a function
of LH treatment time. Bars show mean±s.e.m., and the numbers in
parentheses indicate the number of follicles tested at each time point (0.5
hour=27-40 minutes, 1-2 hours=58-120 minutes).
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