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Figure 5


Fig. 5. Carbenoxolone decreases gap junction permeability and causes meiotic resumption. (A) CBX at a concentration of 100 µm reduces gap junction permeability to an undetectable level. (B) CBX at a concentration of 10 µm reduces gap junction permeability only partially. For A and B, Alexa Fluor 350 was injected into follicle-enclosed oocytes at 58-70 minutes after applying CBX, and follicles were imaged 20 minutes later. A and B are representative of the results of injections into six and 11 follicle-enclosed oocytes, respectively. See Fig. 1A,B for comparison without CBX. (C) A concentration of 100 µm CBX causes NEBD, but 10 µm CBX does not.





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