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Fig. 6. Injection of follicle-enclosed oocytes with an antibody against Cx37
decreases gap junction communication and causes meiotic resumption.
(A) Immunoblot of mouse oocytes (1 µg of protein), showing the
specificity of the Cx37 antibody. (B) An antral follicle in which the
oocyte was injected with 2 µm of the Cx37 antibody, and then, after 5
hours, injected with Alexa Fluor 350; the follicle was imaged 20 minutes
later. B is representative of the results obtained with three antral
follicle-enclosed oocytes. (C) Diffusion of Alexa Fluor 350 from the
oocyte to the somatic cells is inhibited only weakly when tested at 20 minutes
after the Cx37 antibody injection, but is inhibited strongly at 5-6 hours. The
experiments shown in C were carried out using 140-180 µm diameter preantral
follicles, because these provided a technically easier system for
investigating multiple conditions. Somatic cell/oocyte intensity ratios were
measured at 30 minutes after injection of the Alexa Fluor 350; bars show
mean±s.e.m.; n, number of follicles. (D) Injection of
the Cx37 antibody causes NEBD, whereas a control IgG has no effect.
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