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Figure 5


Fig. 5. The incenp mutation delays spindle microtubule assembly in female meiosis. (A) Time taken from nuclear envelope breakdown to the first appearance of spindle microtubules around the chromosomes in wild-type and incenpQA26 mutant oocytes expressing GFP-{alpha}-tubulin. The mean values (bars) are shown with standard deviations (lines). The difference is significant (P<0.001; n≥8). (B) The length of metaphase I-arrested spindles in wild type and the incenpQA26 mutant. (C) The relative amounts of {alpha}-and {gamma}-tubulins in ovaries from wild type and the incenpQA26 mutant were examined by immunoblots. Blotted membranes are stained with MemCode for loading and transfer controls.





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