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Figure 4


Fig. 4. Situs inversus induced by antagonists of Cai2+ asymmetry. Shown are ventral views of embryos treated at HH3++/HH5 with calcium antagonists and analyzed 20 hours later. (A) DMSO control at 15 somites; (B) 30 mM Bapta-AM applied to the left, 14 somites; (C) 1 mM ionomycin applied to the right, 14 somites; (D) 1 µM calmidizolium applied to left, 13 somites; (E) 2.5 mM ryanodine plus 5 mM EGTA applied to left, 12 somites. Heart shape is outlined for clarity.





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