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Fig. 5. Nodal expression after treatment with antagonists of
Cai2+ asymmetry. Embryos received the indicated
agent on the left or the right at HH3++/HH4 and Nodal expression was
evaluated at HH8 or HH9- by using in situ hybridization. Images are dorsal
views with anterior to the top, left (L) and right (R) are as indicated in A;
arrows indicate expression. (A,B) Normal, left-sided
Nodal expression in a DMSO-treated embryo having 3 somites (A) or 5
somites (B). (C) Bilateral Nodal expression in a 3-somite
embryo that received left-sided Bapta-AM. (D) Normal Nodal
expression in 4-somite embryo receiving right-sided Bapta-AM. (E) Loss
of lateral Nodal expression in a 3-somite embryo receiving
right-sided ionomycin (arrow); a faint signal is seen at the left node
(arrowhead). (F) Six-somite embryo receiving left-sided ryanodine plus
EGTA; Nodal expression is absent apart from a small amount to the
left of the tailbud (arrowhead); compare with the 5-somite control embryo in
B. (G) Loss of Nodal expression in a 4-somite embryo that
received left-sided calmidizolium; note Nodal expression in the
tailbud region (arrowhead).
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