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Figure 4


Fig. 4. Ephrin/Eph inhibitors disrupt the sorting of the matrix and striosomal neurons in vitro. (A-F) When mouse E16 LGE-derived cells, enriched for the matrix neurons, are overlaid on postnatal striatal slices preincubated with control Fc reagents (A-C) they display a preference for the DARPP32-negative matrix compartment (arrows). This preference is partially lost when slices are preincubated with soluble ephrin inhibitors EphA3-Fc/EphB2-Fc (D-F), revealing more GFP+ cells in DARPP32-positive compartments (arrowheads). (G-L) When E12-LGE derived cells, enriched for the striosome neurons are overlaid on postnatal striatal slice preincubated with control Fc reagents (G-I), they display a preference for the DARPP32-positive striosome compartments (arrowheads). This preference is partially lost when slices are preincubated with soluble ephrin inhibitors (J-L), revealing more GFP+ cells in DARPP32-negative compartments (arrows). (M) Quantification of the relative densities of GFP+ cells in matrix (M) and striosome (S) compartments. Treatment with ephrin soluble inhibitors results in a decrease of matrix preference for E16 cells, and conversely a decreased striosome preference for E12 cells (*P=0.001, **P=0.0047). Scale bar: 100 µm.





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