QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 2. Targeted disruption of Tshz3. (A) Gene targeting strategy. The mouse Tshz3 locus consists of two exons and one intron, and spans more than 75 kb; the first and second exons encode, respectively, 13 and 1068 amino acids. The in-frame insertion of the lacZ-coding sequence (hatched box) and the neomycin expression cassette (pTK-neo; black box) into exon 2 (ex2; light dotted box) resulted in complete deletion of the zinc-finger motifs and created an XbaI digest size difference between wild-type and targeted loci. Restriction sites are abbreviated as follows: B, BamHI; E, EcoRI; S, SalI, N, NotI, X, XbaI. (B) Southern blot analysis of DNA from wild-type (+/+) and targeted (+/-) ES cell clones using XbaI-digested genomic DNA and a 3' external probe (3' probe; white box in A). (C) Appropriate recombination 5' to the locus was confirmed by PCR with primers shown in A (short arrows). (D) PCR-based genotyping of wild-type (+/+), heterozygous (+/-) and homozygous null (-/-) embryos. (E) Immunostaining of TSHZ3 (left panel) on sections from E15.5 wild-type (+/+) and Tshz3^lacZ/lacZ mutant (-/-) ureter; TSHZ3 is not detected in mutant ureter. Co-immunostaining of TSHZ3 and β-gal (right panel) on a section from E14.5 Tshz3^lacZ/+ heterozygote ureter.

Return to article