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Fig. 3. Tshz3^lacZ/lacZ mice develop congenital hydronephrosis. (A,B) Whole E18.5 urinary tracts. In comparison with wild-type littermates (A), Tshz3^lacZ/lacZ kidneys had bilateral hydronephroses (B). (C) Higher magnification view of proximal hydroureter shown in B. (D-K) Haematoxylin and Eosin-stained transverse sections through E17.5 embryos (D,E) and E18.0 ureters (F-K). Wild-type proximal (F,G) and distal (H) ureter. Tshz3^lacZ/lacZ proximal (I,J) and distal (K) ureter. The mutant ureter shown in I has collapsed post mortem, which is why it does not look dilated. (L-O) Sections are stained with Masson's trichrome and Haematoxylin which results in a red colour for muscles, blue for nuclei and a blue/green for collagens (epithelia also appear red). E16.0 wild-type (L) and mutant (N) ureters with mesenchymal cells surrounding proximal ureter ephithelia indicated by arrowheads. (M,O) High-power magnification images of longitudinal sections from wild-type (L) and Tshz3^lacZ/lacZ (N) E16.0 proximal ureters demonstrate aggregating mesenchyme in wild type and early disorganisation of mesenchymal cells in mutants. (P,Q) E16.5 autopsies in which India ink was injected into the right renal pelvis of a normal (P) and a null mutant (Q) mouse: in both, ink flowed into the bladder (arrowhead). (R) Inset shows dilated proximal ureter filled with ink. bl, bladder; k, kidney; s, stromal layer; u, ureter; ue, ureteric epithelium; um, ureteral mesenchyme; ut, uterus.

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