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Fig. 6. Ureteric SMC differentiation in wild-type and Tshz3-null mutants. (A-L) Transverse sections of E15.5 proximal ureters. In A-F, all nuclei were stained with DAPI (blue) and immunohistochemistry (green positive signals) is shown for SMMHC (A,D), SM22 (B,E) and SMAA (C,F). (G,J) Sections from wild-type (G) or null mutant (J) immunostained for SMAA (green), MYOCD (red) and DAPI (blue); arrow in J indicates MYOCD- and SMAA-positive cells in artery. In situ hybridisation for Myocd (H,K) and Smaa (I,L) (positive purple signal): arrowheads in I and L indicate the Smaa-positive signal in arteries. (M-P) Longitudinal (M,O) and transverse (N,P) sections were counterstained with Haematoxylin (blue) and immunostained for SMAA (brown). In Tshz3 mutant ureter (O) at E17.5, no SMAA was detected in the proximal ureter, in contrast to wild-type ureter (M). Distally, the immunostaining for SMAA was reduced in mutant (P) versus wild-type (N) ureters. (Q-T) E18.5 proximal ureter sections from wild-type (Q,R) or null mutant (S,T) stained with DAPI (blue) and immunostained for RALDH2 (Q,S, red) or UPK (R,T, red). Asterisks indicate the lumen of the ureter; arrowheads in Q and S indicate RALDH2-positive cells.

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