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Fig. 8. Tshz3 induction by BMP4. (A) Whole-mount images of
E12.5 explants (each comprising a metanephros and attached ureter) cultured
for 4 days in absence (left and middle panels) or in presence (right panel) of
recombinant BMP4 (100 ng/ml). Ureters were immunoprobed with antibodies
directed against SMAA (green) and E-cadherin (red; E-Cad) to detect SMC and
epithelia, respectively. In each frame, the proximal ureter is outlined by a
box; note the paucity of SMAA expression in this region in the mutants,
whether or not exposed to exogenous BMP4. (B) Effects of BMP4 on gene
expression in E13.5 wild-type explants cultured for 3 days in absence or
presence of exogenous BMP4 (100 ng/ml). After factoring for Hprt,
real-time Q-PCR revealed significant increases for Id3, Tshz3 and
Myocd. Statistical analyses were performed by one-tailed paired
t-tests. Expression data are represented as mean±s.e.m. with
n=5 in each group.
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